Regulatory
pdps

Part:BBa_K3790014:Design

Designed by: Chongwen Cao   Group: iGEM21_Fudan   (2021-10-01)


The simplest form of dps promoter, a strong σS promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The following primers were used for constructing this part. 

F_dps_1035	5-ACACATAGCCGGTGCTATACTTAATCTCGACTAGAGAAAGAGGAGAAATACTAGATGC
R_dps_1907	5-GTATAGCACCGGCTATGTGTTCCGCTATTCCTCCCACTCCACCTGAACC


Source

The promoter sequence was isolated from E. coli genome.

References

[1] Gisela Becker, Regine Hengge-Aronis. (2001). What makes an Escherichia coli promoter σS dependent? Role of the 213/214 nucleotide promoter positions and region 2.5 of σS. Molecular Microbiology (2001) 39(5), 1153±1165.